Four-dimensional imaging of murine subpleural alveoli using high-speed optical coherence tomography.

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Publication Details

Output type: Journal article

Author list: Kirsten, Gaertner, Schnabel, Meissner, Koch

Publisher: Wiley

Publication year: 2013

Volume number: 6

Issue number: 2

Start page: 148

End page: 152

Number of pages: 5

ISSN: 1864-063X

eISSN: 1864-0648

Languages: English-Great Britain (EN-GB)

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Open access status: closed

Abstract

The investigation of lung dynamics on alveolar scale is crucial for the understanding and treatment of lung diseases, such as acute lung injury and ventilator induced lung injury, and to promote the development of protective ventilation strategies. One approach to this is the establishment of numerical simulations of lung tissue mechanics where detailed knowledge about three-dimensional alveolar structure changes during the ventilation cycle is required. We suggest four-dimensional optical coherence tomography (OCT) imaging as a promising modality for visualizing the structural dynamics of single alveoli in subpleural lung tissue with high temporal resolution using a mouse model. A high-speed OCT setup based on Fourier domain mode locked laser technology facilitated the acquisition of alveolar structures without noticeable motion artifacts at a rate of 17 three-dimensional stacks per ventilation cycle. The four-dimensional information, acquired in one single ventilation cycle, allowed calculating the volume-pressure curve and the alveolar compliance for single alveoli.

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