TLC-Bioautography and GC-MS Analyses for Detection and Identification of Antioxidant Constituents of Trachyspermum copticum Essential Oil
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Publication Details
Output type: Journal article
Author list: Nickavar B, Adeli A, Nickavar A
Publisher: School of Pharmacy, Shahid Beheshti University of Medical Sciences
Publication year: 2014
Volume number: 13
Issue number: 1
Start page: 127
End page: 133
Number of pages: 7
ISSN: 1735-0328
Languages: English-Great Britain (EN-GB)
Abstract
The present work was designed to study the antioxidant activity and to identify the main active components of the essential oil of ajowan (Trachyspermum copticum) fruit. GC and GC-MS analyses of the essential oil showed the presence of eight compounds. The main constituents of the oil were thymol (43.7%), p-cymene (26.8%), and gamma-terpinene (24.9%). The antioxidant and free radical scavenging activities of ajowan oil was evaluated by using ABTS(center dot+) and beta-carotene bleaching assays. The oil exhibited a considerable dose-dependent antioxidant activity. Antioxidant activity guided fractionation of the oil was carried out by TLC-bioautography method based on the DPPH center dot assay to screen and separate the main active constituents. The bioautography screening and fractionation resulted in the separation of the main antioxidant compound which was identified as thymol.
Keywords
Antioxidant activity, Chemical composition, Essential oil, Trachyspermum copticum
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