Sulforaphane induces growth arrest and apoptosis in human ovarian cancer cells.

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Output type: Journal article

Author list: Chuang, Moqattash, Gretz, Nezhat, Rahaman, Chiao

Publisher: Wiley Open Access

Publication year: 2007

Journal: Acta Obstetricia et Gynecologica Scandinavica (0001-6349)

Volume number: 86

Issue number: 10

Start page: 1263

End page: 8

Number of pages: -1254

ISSN: 0001-6349

eISSN: 1600-0412

Languages: English-Great Britain (EN-GB)

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Open access status: bronze

Full text URL: https://onlinelibrary.wiley.com/doi/pdfdirect/10.1080/00016340701552459

Abstract

OBJECTIVES\nIsothiocyanates (ITC) from broccoli and other cruciferous vegetables have long been shown to have chemopreventive properties, as demonstrated in cancer models in rodents. Sulforaphane (SFN) is a major ITC present in broccoli. We examined the effects of SFN on the growth of the OVCAR-3 and SKOV-3 ovarian carcinoma cell lines.\nMETHODS\nCell cycle phase determination was performed using a Coulter flow cytometer. DNA strand breaks in apoptotic cells were measured by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end-labelling (TUNEL).\nRESULTS\nThere was a concentration dependent decrease in cell density. Approximately 50% decrease was observed after 48 h of incubation with SFN (2 μM). Analysis of cell cycle phase progression revealed a decrease in the cell populations in S and G2M phases, with an increase of G1 cell population, indicating a G1 cell cycle arrest. The degree of decrease in the replicating population was concentration and time dependent. Incubation of OVCAR-3 cells in cultures with concentrations of 2, 10 and 50 μM of SFN showed 6, 8 and 17% apoptosis, respectively. In addition, when OVCAR-3 cells were exposed to SFN for various time periods (1, 2 or 3 days), the percentage of cells undergoing apoptosis was directly proportional to the incubation period. In this regard, while 18% of the cells underwent apoptosis after 2 days, 42% of the cells showed apoptosis after 3 days of incubation.\nCONCLUSIONS\nThese results clearly demonstrated an effect of SFN in inducing growth arrest and apoptosis in ovarian carcinoma cell lines.

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