The properties of a mammary gland cancer stem cell
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Publication Details
Output type: Journal article
Author list: Zucchi I, Sanzone S, Astigiano S, Pelucchi P, Scotti M, Valsecchi V, Barbieri O, Bertoli G, Albertini A, Reinbold RA, Dulbecco R
Publisher: National Academy of Sciences
Publication year: 2007
Journal: Proceedings of the National Academy of Sciences (0027-8424)
Volume number: 104
Issue number: 25
Start page: 10476
End page: 10481
Number of pages: 6
ISSN: 0027-8424
eISSN: 1091-6490
Languages: English-Great Britain (EN-GB)
Unpaywall Data
Open access status: green
Full text URL: https://europepmc.org/articles/pmc1965538?pdf=render
Abstract
The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing a single cancer-initiating cell with stem cell properties has proven technically difficult because of the scarcity of the cancer stem cells in the tissue of origin and the lack of specific markers for cancer stem cells. Here we show that a single LA7 cell derived from rat mammary adenocarcinoma has the following properties: the differentiation potential to generate all of the cell lineages of the mammary gland; the ability to generate branched duct-like structures that recapitulate morphologically and functionally the cluctal-alveolar-like architecture of the mammary tree; and the capacity to initiate heterogeneous tumors in nonobese diabetic-SCID mice. In addition, we show that cultured cells derived from tumors generated by a single LA7 cell-injection have properties similar to LA7 cells, can generate all of the cell lineages of the mammary gland, and recapitulate the ductal-alveolar-like architecture of the mammary tree. The properties of self-renewal, extensive capacity for proliferation, multilineage differentiation potential, and single-cell tumor-initiation potential suggest that LA7 cells are cancer stem cells and can be used as a model system to study the dynamics of tumor formation at the single-cell level.
Keywords
mammary gland differentiation, p21/(WAF1), single cell injection
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