Association of rat8 with Fyn protein kinase via lipid rafts is required for rat mammary cell differentiation in vitro
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Output type: Journal article
Author list: Zucchi I, Prinetti A, Scotti M, Valsecchi V, Valaperta R, Mento E, Reinbold R, Vezzoni P, Sonnino S, Albertini A, Dulbecco R
Publisher: National Academy of Sciences
Publication year: 2004
Journal: Proceedings of the National Academy of Sciences (0027-8424)
Volume number: 101
Issue number: 7
Start page: 1880
End page: 1885
Number of pages: 6
ISSN: 0027-8424
eISSN: 1091-6490
Languages: English-Great Britain (EN-GB)
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Open access status: green
Full text URL: https://europepmc.org/articles/pmc357021?pdf=render
Abstract
We previously identified rat8 in the pathway involved in epithelial cell differentiation that occurs in the rat mammary gland at pregnancy when tubules and alveoli are formed. rat8, which encodes an IFN-inducible membrane protein, is the rat homologue of the mouse gene fragilis. By differential detergent extraction and isopycnic sucrose density gradients, we show that rat8 protein is associated to lipid membrane domains together with Lyn and Fyn, members of the Src tyrosine kinase family. We also show that recruitment of rat8 to lipid membrane domains is a necessary step in mammary epithelial cell differentiation. Immunoprecipitation analysis, performed with an anti-Fyn protein antibody, shows that rat8 was present in the Fyn immunoprecipitate. Antisense oligonucleotides, used to inhibit Fyn protein expression, block mammary cell differentiation. Taken together, these results suggest that the functional interaction, via lipid membrane domains, of rat8 and Fyn proteins is required for mammary cell differentiation. Therefore, rat8, like fragilis, may be involved in developmental decisions and the demarcation of a subset of cells in the mammary gland that cause epithelial cells to develop into a network of tubuloalveolar structures involved in secretion.
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