Thyroid hormone responsive Spot 14 increases during differentiation of human adipocytes and its expression is down-regulated in obese subjects

Authors/Editors

Research Areas

Publication Details

Output type: Journal article

Author list: Ortega F., Vazquez-Martin A., Moreno-Navarrete J., Bassols J., Rodriguez-Hermosa J., Gironés J., Ricart W., Peral B., Tinahones F., Fruhbeck G., Menendez J., Fernandez-Real J.

Publisher: Springer Nature [academic journals on nature.com]

Publication year: 2010

Journal: International Journal of Obesity (0307-0565)

Volume number: 34

Issue number: 3

Start page: 487

End page: 499

Number of pages: 13

ISSN: 0307-0565

eISSN: 1476-5497

URL: http://api.elsevier.com/content/abstract/scopus_id:77949541352

• View in Web of Science
•  | 
• View on publisher site
•  | 
• View citing articles in Web of Science

Unpaywall Data

Open access status: bronze

Full text URL: https://www.nature.com/articles/ijo2009263.pdf

Abstract

Context:Very limited information is available regarding the function of human thyroid hormone responsive Spot 14 (human S14, hS14) in adipogenesis and human adiposity.Objective:To evaluate hS14 levels during differentiation of human pre-adipocytes, in human fat depots and isolated fat cells.Design:This was a cross-sectional study.Subjects:A total of 161 omental (OM) and 87 subcutaneous (SC) adipose tissue samples obtained during elective surgical procedures from a population who varied widely in terms of obesity.Measurements: hS14 gene expression and protein levels during adipogenesis were assessed by RT-PCR, western blot, and using an automated confocal imaging approach.Results:hS14 gene expression levels were decreased in OM adipose tissue from overweight (-42.0%) and obese subjects (56.5%) compared with lean subjects (P<0.05 and P<0.0001, respectively). hS14 mRNA (but not hS14-related) was inversely associated with obesity measures such as body mass index (P=0.001), percent fat mass (P=0.001), waist-to-hip ratio (P=0.020), and systolic blood pressure (P=0.031). hS14 gene expression and protein levels were up-regulated at the early stages of differentiation of human pre-adipocytes as well as for 3T3-L1 cells. That observation was most prominent in those individual cells exhibiting the more marked differentiation features. hS14 gene expression levels increased by ∼45 000-fold in mature adipocytes. Increased hS14 levels were also found in stromal-vascular cells/pre-adipocytes (3.8-fold, P<0.05) and in adipose tissue samples (1.9-fold, P<0.0001) from SC compared with OM fat depots.Conclusions:These results suggest that hS14 is involved in human adipogenesis, but inversely related to obesity and OM fat accumulation. © 2010 Macmillan Publishers Limited All rights reserved.

Keywords

Adipogenesis, Adipose tissue, Gene expression, Mature adipocytes, Pre-adipocytes, Thyroid hormone responsive Spot 14

Documents

No matching items found.