Proadipogenic effects of lactoferrin in human subcutaneous and visceral preadipocytes

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Publication Details

Output type: Journal article

Author list: Moreno-Navarrete J., Ortega F., Sabater M., Ricart W., Fernández-Real J.

Publisher: Elsevier

Publication year: 2011

Journal: Journal of Nutritional Biochemistry (0955-2863)

Volume number: 22

Issue number: 12

Start page: 1143

End page: 1149

Number of pages: 7

ISSN: 0955-2863

eISSN: 1873-4847

URL: http://api.elsevier.com/content/abstract/scopus_id:80655145285

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Open access status: closed

Abstract

Lactoferrin has been associated with insulin sensitivity in vivo and in vitro studies. We aimed to test the effects of lactoferrin on human subcutaneous and visceral preadipocytes. Human subcutaneous and visceral preadipocytes were cultured with increasing lactoferrin (hLf, 0.1, 1, 10 μM) under differentiation conditions. The effects of lactoferrin on adipogenesis were studied through the expression of different adipogenic and inflammatory markers, AMPK activation and Retinoblastoma 1 (RB1) activity. The response to insulin was evaluated through AKT phosphorylation. In both subcutaneous and visceral preadipocytes, lactoferrin (1 and 10 μM) increased adipogenic gene expressions and protein levels (fatty acid synthase, PPARγ, FABP4, ADIPOQ, ACC and STAMP2) and decreased inflammatory markers (IL8, IL6 and MCP1) dose-dependently in parallel to increased insulin-induced AKT phosphorylation. In addition to these adipogenic effects, lactoferrin decreased significantly AMPK activity (reducing AMPK and ACC) and RB1 activity (increasing the RB1/RB1 ratio). In conclusion, these results suggest that lactoferrin promotes adipogenesis in human adipocytes by enhancing insulin signaling and inhibiting RB1 and AMPK activities. © 2011 Elsevier Inc.

Keywords

Adipogenesis and insulin action, Lactoferrin

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