Liver-type fatty acid binding protein interacts with hepatocyte nuclear factor 4 alpha

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Publication Details

Output type: Journal article

Author list: McIntosh AL, Petrescu AD, Hostetler HA, Kier AB, Schroeder F

Publisher: Wiley

Publication year: 2013

Journal: FEBS Letters (0014-5793)

Volume number: 587

Issue number: 23

Start page: 3787

End page: 3791

Number of pages: 5

ISSN: 0014-5793

eISSN: 1873-3468

Languages: English-Great Britain (EN-GB)

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Open access status: bronze

Full text URL: https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2013.09.043

Abstract

Hepatocyte nuclear factor 4 alpha (HNF4 alpha) regulates liver type fatty acid binding protein (L-FABP) gene expression. Conversely as shown herein, L-FABP structurally and functionally also interacts with HNF4 alpha. Fluorescence resonance energy transfer (FRET) between Cy3-HNF4 alpha (donor) and Cy5-L-FABP (acceptor) as well as FRET microscopy detected L-FABP in close proximity (similar to 80 angstrom) to HNF4 alpha, binding with high affinity K-d similar to 250-300 nM. Circular dichroism (CD) determined that the HNF4 alpha/L-FABP interaction altered protein secondary structure. Finally, L-FABP potentiated transactivation of HNF4 alpha in COS7 cells. Taken together, these data suggest that L-FABP provides a signaling path to HNF4 alpha activation in the nucleus. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Keywords

Fatty acid binding protein, Hepatocyte nuclear factor 4 alpha, Liver

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