Quantum dot-based immunoassay enhanced by high-density vertical ZnO nanowire array.
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Publication Details
Output type: Journal article
Author list: Kim, Kwon, Park, Park
Publisher: Elsevier
Publication year: 2014
Journal: Biosensors and Bioelectronics (0956-5663)
Volume number: 55
Start page: 209
End page: 15
Number of pages: -193
ISSN: 0956-5663
Languages: English-Great Britain (EN-GB)
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Open access status: closed
Abstract
In this paper, we report an efficient and high-performance immunoassay platform by combining high-density vertical ZnO nanowire array with photostable quantum dot (QD) labeling. The ZnO nanowire array provides a large surface area for the immobilization of biomolecules, which makes it an efficient substrate for the immunoreaction of biomolecules. When a sandwich immunoassay with QD label was conducted on various substrates, the ZnO nanowire substrate showed stronger fluorescence signal than ZnO thin film and bare glass substrates by 3.8 and 8.5 times, respectively. We found that the fluorescence resonance energy transfer (FRET) from QD to ZnO nanowire could be suppressed by extending their distance with multilayer biotin-streptavidin complex. In addition, we demonstrated the QD-based immunoassay of carcinoembryonic antigen (CEA) on a ZnO nanowire substrate, showing an excellent immunoassay performance with a very low detection limit (0.001ng/mL) and a large detection range up to 100ng/mL.
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