Development and validation of ethylenethiourea determination in foods using methanol-based extraction, solid-phase extraction cleanup and LC-MS/MS.
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Output type: Journal article
Author list: Tran K, Mactal LP, Cromer MR, Vocque RH, Smith RE, asölfjkaöls kdfjadf,asdfas
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Publisher: Elsevier
Publication year: 2013
Journal: Food Chemistry (0308-8146)
Volume number: 140
Issue number: 1-2
Start page: 340
End page: 352
Number of pages: 13
ISSN: 0308-8146
URL: http://www.ncbi.nlm.nih.gov/pubmed/23578651
Languages: English-Great Britain (EN-GB)
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Open access status: closed
Abstract
To response to the need for a rapid, cost-effective, eco-friendly and efficient extraction process, a sensitive method for the determination of ethylenethiourea (ETU) in food matrices by high-performance liquid chromatography-mass spectrometry (HPLC-LC/MS) was developed and validated. ETU was extracted from food commodities with methanol, cleaned up by alumina-SPE column, and then determined by HPLC-MS/MS. The MS detection was operated in positive ionization mode. For confirmation of target compound, two precursor ion>product ion transitions were selected by multi-reaction monitoring mode (MRM). The method showed good linearity with correlation coefficient higher than 0.9950. Recoveries at three spiked levels (10, 50, 100ng/g) in random selected food matrices were in range of 71-121% with RSDs not larger than 25%. The limit of quantitation for the analyte was estimated at 5ng/g.
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