Proteomic dissection of dome formation in a mammary cell line
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Publication Details
Output type: Journal article
Author list: Zucchi I, Dulbecco R
Publisher: Springer
Publication year: 2002
Journal: Journal of Mammary Gland Biology and Neoplasia (1083-3021)
Volume number: 7
Issue number: 4
Start page: 373
End page: 384
Number of pages: 12
ISSN: 1083-3021
eISSN: 1573-7039
Languages: English-Great Britain (EN-GB)
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Open access status: closed
Abstract
The study of the development of the mammary gland at the molecular level in animals is difficult because of the complex tissue organization. This review introduces a proteomic approach to investigate mammary gland development in a cell culture system that we have previously developed as an in vitro model for studying mammary cell differentiation. The model is based on two cell lines, one of which is able to differentiate spontaneously and produce hemispherical blisters, called domes, when confluent. Through proteomic dissection of dome-forming cells, two types of key regulatory genes have been identified: genes inducing cellular structural modifications and genes related to functional modifications. We identified several genes in the pathway leading to dome formation in vitro and showed that the functional and structural changes taking place in dome-forming cells correspond to cellular changes occurring in vivo when tubules and alveoli are developed in the mammary gland at pregnancy.
Keywords
dome formation, ENaC-beta-subunit, mammary gland differentiation, maspin, proteome, rat8
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