Allergen arrays for antibody screening and immune cell activation profiling generated by parallel lipid dip-pen nanolithography.
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Publication Details
Output type: Journal article review
Author list: Sekula-Neuner S, Maier J, Oppong E, Cato AC, Hirtz M, Fuchs H
Publisher: Wiley
Publication year: 2012
Journal: Small (1613-6810)
Volume number: 8
Issue number: 4
Start page: 585
End page: 91
Number of pages: -493
ISSN: 1613-6810
eISSN: 1613-6829
Languages: English-Great Britain (EN-GB)
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Open access status: closed
Abstract
Multiple-allergen testing for high throughput and high sensitivity requires the development of miniaturized immunoassays that allow for a large test area and require only a small volume of the test analyte, which is often available only in limited amounts. Developing such miniaturized biochips containing arrays of test allergens needs application of a technique able to deposit molecules at high resolution and speed while preserving its functionality. Lipid dip-pen nanolithography (L-DPN) is an ideal technique to create such biologically active surfaces, and it has already been successfully applied for the direct, nanoscale deposition of functional proteins, as well as for the fabrication of biochemical templates for selective adsorption. The work presented here shows the application of L-DPN for the generation of arrays of the ligand 2,4-dinitrophenyl[1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[6-[(2,4-dinitrophenyl)amino]hexanoyl] (DNP)] onto glass surfaces as a model system for detection of allergen-specific Immunoglobin E (IgE) antibodies and for mast cell activation profiling.
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